RNA

Part:BBa_K4409017:Design

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-26)


guide of igRNA (GAPDH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to be a guide sequence for the igRNA because GAPDH is a very good candidate for the substrate dsDNA used in our CRISPR-Cas12 system. Substrate dsDNA is used to activate the Cas12 enzyme. A large amount of dsDNA is needed in the system to achieve sensitive signals. GAPDH is abundant in cells and can be easily amplified, so GAPDH dsDNA can be easily obtained in large quantity. To form an igRNA, this part can be coupled with our trigger-RNA sensor (BBa_K4409004) which contains a loop sequence complementary to it, so that the loop sequence can block this part when no trigger is in the system.


Source

This part is based on the GAPDH gene sequence retrieved from Ensembl database.

References